Skip to main content
. 2009 Dec 15;159(3):543–553. doi: 10.1111/j.1476-5381.2009.00533.x

Figure 3.

Figure 3

Effect of bisindolylmaleimide I and DMS on FCS- and PMA-stimulated ERK-1/2 activation. PVSMCs were pretreated with and without bisindolylmaleimide I (Bis; 0.1–10 µM or DMS (50 µM for 10 min, and then stimulated with either PMA (1 µM or FCS (10%, v/v) for 10 min. (A) Western blot showing the comparative effect of DMS and bisindolylmaleimide I on PMA- and FCS-stimulated ERK-1/2 activation; (B) Western blots showing the concentration-dependent effect of bisindolylmaleimide I on PMA, but not FCS-stimulated ERK-1/2 activation. Phosphorylated ERK-1/2 (P-ERK-1/2) was detected on Western blots probed with anti-phospho ERK-1/2 antibody. Blots were also probed with anti-ERK-2 antibody to ensure equal protein loading. Representative blots are shown. DMS/PMA (n = 18), DMS/FCS (n = 10), Bis/PMA (n = 14), Bis/FCS (n = 11).