Figure 5.

Effect of tranilast on mouse mast cell protease (mMCP)4, mMCP6, stem cell factor (SCF), tumour necrosis factor (TNF)-α, interleukin (IL)-6 and IL-10 mRNA levels in the inflamed paws of collagen-induced arthritic mice. Competitive RT-PCR was used to determine mRNA levels of mMCP4 (A), mMCP6 (B), SCF (C), TNF-α (D), IL-6 (E) and IL-10 (F) in non-immunized normal control mice (Normal), vehicle-treated collagen-induced arthritic mice (Vehicle) and tranilast-treated (Tranilast) collagen-induced arthritic mice. These mRNA levels were higher in inflamed paws than in normal paws. The increase of these mRNA levels except for IL-10 was inhibited by tranilast treatment. IL-10 mRNA level was increased by tranilast treatment. Results are shown as the mean ± SEM for 12 animals in each group. *P < 0.05.