Figure 4.

TRAM-34 competes with [³H]-17β-oestradiol (E2) for binding to the oestrogen receptor (ER). Data are expressed as a percentage of vehicle control ± standard error of the mean. Unlabelled E2 (0.01 µM) reduced the binding of [³H]-E2 by ∼50% (P < 0.05, n= 4). TRAM-34 (at low concentrations, 0.01–0.1 µM) did not reduce the binding of [³H]-E2. These data were determined from four sample replicates from one protein extraction, therefore only n= 1. Statistical analysis (Student's t-test) performed on this one protein extraction comparing the mean of four control samples versus four TRAM-34 samples yielded a P > 0.05, and was determined to be not statistically significant. TRAM-34 at higher concentrations (1–100 µM) significantly reduced the specific binding of [³H]-E2 (P < 0.05, n= 3 independent protein extractions). *P < 0.05 compared with vehicle control.