Figure 5.

Effects of insulin on the dexamethasone-induced phosphorylation of n-myc downstream regulated protein 1 (NDRG1)-Thr^346/356/366 in H441 cells. All cells were cultured in nominally hormone-free medium for 6 days before being used in experiments. (A) Typical Western blots showing the changes to the cellular abundance of total and Thr^346/356/366-phosphorylated NDRG1 evoked by acute (0–6 h) exposure to 0.2 µM dexamethasone. (B) Directly analogous blots showing the changes in total and Thr^346/356/366-phosphorylated NDRG1 abundance evoked by stimulation (0–6 h) with a combination of 0.2 µM dexamethasone and 20 nM insulin. (C) Pooled data (n= 5) showing the changes in the abundance of Thr^346/356/366-phosphorylated (upper panel) and total (lower panel) NDRG1 measured in cells that had been stimulated with 0.2 µM dexamethasone, either alone (Dex.) or in combination with 20 nM insulin (Dex./Ins.).