Figure 7.

Insulin-induced n-myc downstream regulated protein 1 (NDRG1)-Thr^346/356/366 phosphorylation in mpkCCD cells. Cells were stimulated with insulin (20 nM) for 0–6 h before cellular protein was extracted for Western analysis. (A) Typical blot showing the changes in the cellular abundance of Thr^346/356/366-phosphorylated and total NDRG1 that occur during exposure to insulin. (B) Pooled data (n= 5) showing the insulin evoked changes to the cellular abundance of Thr^346/356/366-phosphorylated and total NDRG1. CCD, cortical collecting duct.