Figure 8.

Effects of peroxisome proliferator-activated receptor gamma (PPARγ) agonists on serum and glucocorticoid-inducible kinase 1 (SGK1) activity in mpkCCD cells. (A) Typical blots showing the effects of insulin stimulation upon the cellular abundance of Thr^346/356/366-phosphorylated and total n-myc downstream regulated protein 1 (NDRG1) in control and pioglitazone-treated (10 µM, 4 h) cells. (B) Pooled data showing effects of insulin upon the phosphorylation of NDRG1-Thr^346/356/366 in control and pioglitazone-treated cells (n= 6). (C) Typical blots showing insulin-induced phosphorylation of NDRG1-Thr^346/356/366 in control and rosiglitazone-treated (2 µM, 4 h) cells. (D) Pooled data showing insulin-evoked phosphorylation of NDRG1- Thr^346/356/366 in control and rosiglitazone-treated cells (n= 6). **P < 0.002; ***P < 0.001, significant effects of insulin; †P < 0.05, significant effect of pioglitazone; Student's paired t-test. CCD, cortical collecting duct.