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Michaelis–Menten kinetics of BSP net uptake mediated by OATP1B3 WT and the mutants Lys41>Ala, Lys41>Arg, Arg580>Gly and Arg580>Lys. The uptake of BSP was measured at 10 min. The Vmax values are normalized to cell surface protein expression of OATP1B3 WT/mutants. Data are presented as mean ± SEM. ***Significantly different compared to WT, P < 0.001.
