Figure 4.

Structure of the CHUP1 Gene and Positions of chup1 Mutations.

The locations of start (ATG) and stop (TGA) codons are indicated. Nine exons (boxes) and eight introns (lines) were identified by comparing the genomic sequence with the cDNA sequence. Mutations in chup1-1, chup1-2, chup1-3, chup1-4, chup1-5, and chup1-6 are as follows: insertion of T-DNA into exon 5 in chup1-1; the codon for Gln-234 was changed to a stop codon in chup1-2; the codon for Gln-75 was changed to a stop codon in chup1-3; the codon for Trp-451 was changed to a stop codon in chup1-4; the codon for Trp-828 was changed to a stop codon in chup1-5; and the first base (G) of intron 8 was changed to A in chup1-6.