Table 1. Patient characteristics.

The information on the pre- and post-biopsy parameters was assessed by pathologic examination of the surgical specimens (Gleason sum (biopsy or radical prostatectomy, margin status, extra prostatic extension (EPE), seminal vesicle invasion and lymph node status) and the available clinical information (age, PSA and clinical stage). In this study all patients were node negative. Therefore, these parameters are considered as baseline covariates. Mean ± SD staining scores for HA and HYAL1 are shown.

Characteristics	Recurred	Non-Recurred
Number of patients	23	38
Age	Mean: 62.4	Mean: 61.1
	Median: 65; 95% CI: 57 – 66	Median: 63; 95% CI: 59 – 65
Parameters available at biopsy stage
PSA	Mean: 12.8	Mean: 8.3
	Median: 13; 95% CI: 6.7 – 15.7	Median: 7.3; 95% CI: 6 – 8.4
Gleason sum	GL6=10	GL6=28
	GL7=5	GL7=9
	GL8=4	GL9=1
	GL9=4
Clinical Stagea	T1C=14	T1C=20
	T2A=5	T2A=6
	T2B=3	T2B=10
	T3=1	T3=2
HA staining	182.1 ± 94.1	87 ± 75.8
HYAL1 staining	203.9 ± 88.6	48.8 ± 75.7
Parameters available after prostatectomy
Gleason sum	GL5= 0	GL5=2
	GL6= 4	GL6=14
	GL7= 8	GL7=15
	GL8= 6	GL8=6
	GL9= 5	GL9=1
EPE	(+) 14	(+) 4
	(−) 9	(−) 34
Pathologic Stage	T2 = 6	T2 = 34
	T3a = 7	T3a = 1
	T3b = 10	T3b = 3
Seminal vesicle invasion	(+) 10	(+) 3
	(−) 13	(−) 35
Margin	+) 14	(+) 8
	(−) 9	(−) 30
HA staining	236 ± 81.1	125 ± 96.4
HYAL1 staining	266.3 ±53	86.7 ± 89.5