Fig. 2.

cpg15 and cpg15-2 are both activity-regulated genes. A: cpg15 and cpg15-2 expression in the brain is induced by kainate. cpg15 and cpg15-2 expression in cerebral cortices of uninjected (—) and kainate-injected (KA) mice was examined by Northern blot analysis. The blot was reprobed with a gapdh probe as a loading control. B: Quantitative measurement of kainate-induced cpg15 and cpg15-2 expression by real-time quantitative reverse transcription polymerase chain reaction (RT-PCR). Expression level of each gene was normalized to gapdh expression levels. Fold induction of each gene relative to its basal expression level in uninjected controls is plotted. Data are represented as mean ± standard error of mean. **P < 0.01, n = 5 for KA, n = 3 for controls. C: Induction by light of cpg15 and cpg15-2 in visual structures. Mice were placed in the dark for 4 weeks, then either exposed to light for 24 hours or kept in the dark. cpg15 and cpg15-2 expression in the retina and the visual cortex was measured by real-time RT-PCR and plotted as in B. *P < 0.05, **P < 0.01, n = 3.