Fig. 5.

CPG15 and CPG15-2 are expressed by different cell types in the retina. A: CPG15-2 immunohistochemistry. Retinal sections were immunostained with the anti-CPG15-2 antibody (magenta), then counterstained with 4′,6-diamidino-2-phenylindole (DAPI; green). CPG15-2 is localized to the outer plexiform layer, inner nuclear layer, and inner plexiform layer. Anti-CPG15-2 staining was competed out by the addition of a CPG15-2 peptide but not by a CPG15 peptide. B: PKCα-positive rod bipolar cells express CPG15-2. Dual staining of the retina with anti-CPG15-2 (magenta) and anti-PKCα (green) antibodies shows colocalization of CPG15-2 with PKCα (white) in the axons of rod bipolar cells. Not all CPG15-2-stained fibers are PKCα positive, as indicated by the arrows. Maximum intensity projections of five confocal images taken at 1-μm intervals are shown. C: In situ hybridization on retinal sections probed for cpg15. cpg15 signal can be seen in the ganglion cell layer. Signal in the pigment epithelium is due to nonspecific light scattering. PE, pigment epithelium; ONL, outer nuclear layer; OPL, outer plexiform layer; INL, inner nuclear layer; IPL, inner plexiform layer; GCL, ganglion cell layer. Scale bars = 50μm.