Fig. 1.

The diurnal rhythm of wakefulness, non-rapid-eye-movement sleep (NREMS), rapid-eye-movement sleep (REMS), electroencephalographic (EEG) slow-wave activity (SWA) during NREMS, body temperature, and locomotor activity of wild-type [WT (left), n = 8] and preproghrelin knockout [KO (right); sleep data: n = 8 ; temperature and activity data: n = 11] mice on the baseline days (open symbols) and on the restricted feeding (RF, filled symbols) days 1–3. Data are averaged across RF days 1, 2, and 3 and expressed in 1-h bins for each parameter except for EEG SWA where data points represent 2-h averages. Error bars, SE; gray shaded area, dark phase of the day. *Significant difference between baseline days and treatment days (univariate tests of significance for planned comparison, P < 0.05).