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. 2003 Dec;15(12):2900–2910. doi: 10.1105/tpc.014712

Figure 6.

Figure 6.

Expression Pattern of D2/CYP90D2 and CYP90D3 in Various Organs, and the Negative Feedback Effect of BL on D2/CYP90D2 Expression.

(A) Organ-specific expression of D2/CYP90D2 and CYP90D3 in a wild-type plant. Total RNA was isolated from root (RT), flower (FL), panicle (PA), shoot apex (SA), leaf blade (LB), leaf sheath (LS), elongating stem (ST1), and elongated stem (ST2), and RT-PCR was performed. Signals were detected with the 32P-labeled cDNA clone indicated at left. Expression of the Actin gene was used as a control.

(B) Negative feedback regulation of D2/CYP90D2 by BL. Total RNA was prepared from 10-day-old seedlings of the wild type (WT), brd1-1, brd1-2, and d61-2 with or without exogenous application of 10−6 M BL. Expression of the Actin gene was used as a control.