FIG. 1.

SAHA-induced activation of 24STNLESG cells. (A) 24STNLESG cells (2 × 10⁵) were plated in 24-well plates. Twenty-four hours later they were treated as indicated and assayed for SEAP activity 48 h posttreatment. Experiments were performed in triplicate and the results are presented relative to the values obtained for DMSO treatment. (B) The percentage of reactivated 24STNLSG cells was evaluated by flow cytometry 48 h on SAHA (5.0 μM) or VPA (1.0 mM) treatment. Latent reflects an untreated control. Data from one of two representative experiments are shown. (C) Individual clones of HeLa cells infected with the NLRLucRFP vector were untreated, treated with 1.0 mM valproic acid, or treated with 2.0 μM SAHA, and then assayed for activity. The experiment was performed in triplicate and results are presented as relative light units (RLU) as measured by the luminometer. Results in (A) and (C) are presented as ± SEM.