FIG. 2.

SAHA causes chromatin acetylation and remodeling at the HIV-1 LTR. (A) 24STNLESG cells (1 × 10⁷) were treated with DMSO, valproic acid (1 mM), or SAHA (5 μM). Chromatin was extracted and precipitated with no antibody, anti-acH3, or anti-acH4. Precipitated DNA was recovered and quantified by real-time PCR performed in triplicate. Results are presents as percentage of DNA recovered relative to the input amount, p < 0.05. (B) 24STNLESG cells (1 × 10⁷) were treated as described. The nuclei were isolated and then treated with 60 U micrococcal nuclease for 5 min. Genomic DNA was recovered and quantified by real-time PCR performed in triplicate, p < 0.05. Results are presented as amount of DNA recovered relative to undigested nuclei, normalized to untreated cells.