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. Author manuscript; available in PMC: 2011 Jan 29.
Published in final edited form as: Biochem Biophys Res Commun. 2010 Jan 6;392(1):72–76. doi: 10.1016/j.bbrc.2009.12.171

Figure 1.

Figure 1

A) Increased OPG and decreased RANKL mRNA expression levels in TIEG KO osteoblasts. Calvarial osteoblasts were isolated from three individual WT and KO littermate pups and cultured in vitro. Total RNA was isolated from these cells and real-time PCR analysis was performed to measure OPG and RANKL mRNA levels. The results are expressed as fold change relative to WT cells. The results were repeated in three independent experiments and a representative data set is shown. * indicates significance at the P<0.05 level (ANOVA) compared to WT cells. B) Transient transfection analysis of TIEG mediated OPG and RANKL promoter activity in calvarial osteoblasts. TIEG KO calvarial osteoblasts were transiently transfected with 500 ng of either the OPG or RANKL promoter construct in combination with 500 ng of either the pcDNA4/TO empty vector or pcDNA4/TO-TIEG expression construct. Twenty-four hours following transfection, cells were lysed and analyzed for luciferase activity. The results were normalized to empty vector controls. The results were repeated in three independent experiments and a representative data set is shown. * indicates significance at the P<0.05 level (ANOVA) compared to vector controls.