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. Author manuscript; available in PMC: 2011 Jan 29.
Published in final edited form as: Biochem Biophys Res Commun. 2010 Jan 6;392(1):72–76. doi: 10.1016/j.bbrc.2009.12.171

Figure 4. Identification of Sp-1 sites important for TIEG regulation of OPG promoter activity.

Figure 4

A) Four potential Sp-1 binding sites were identified within the first 200 bps of the OPG promoter. B) Site-directed mutagenesis was used to alter each of the identified Sp-1 binding sites. pGL3 basic luciferase constructs harboring the full length OPG promoter with or without individually mutated Sp-1 sites were transfected into TIEG KO calvarial osteoblasts in the presence or absence of a TIEG expression construct. Twenty-four hours following transfection, cells were lysed and analyzed for luciferase activity. The results were repeated in three independent experiments and a representative data set is shown. * indicates significance at the P<0.05 level (ANOVA) compared to vector controls. # indicates significance at the P<0.05 level (ANOVA) compared to the full-length OPG promoter.