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. Author manuscript; available in PMC: 2010 Feb 24.
Published in final edited form as: Mol Pharmacol. 2008 Apr 3;74(1):20–33. doi: 10.1124/mol.108.047035

Fig. 3.

Fig. 3

Enumeration of lymphocyte subpopulations in the liver of ConA injected mice. Mice (four/group) were injected with vehicle, THC, ConA + vehicle, or ConA + THC (50 mg/kg). After 12 h, hepatic MNCs were harvested, and cells were stained for various markers and analyzed by fluorescence-activated cell sorting. A, representative double-stain flow profiles showing percentages of each cell population. B, absolute cell numbers of various lymphocyte subpopulations were calculated by taking into consideration total number of liver MNCs isolated per mouse and percentage of cells as shown in A (†, p < 0.05 compared with vehicle; *, p < 0.05 compared with ConA). C, detection of apoptosis in liver MNCs using TUNEL assay. MNCs isolated 12 h after ConA injection were analyzed by TUNEL for apoptotic cells by flow cytometry. Histograms represent TUNEL staining on CD3+ cells. The last panel shows an overlay of three histograms.