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. Author manuscript; available in PMC: 2010 Feb 24.
Published in final edited form as: Mol Pharmacol. 2008 Apr 3;74(1):20–33. doi: 10.1124/mol.108.047035

Fig. 4.

Fig. 4

Murine hepatocyte cells are resistant to THC-induced apoptosis. Normal murine hepatocyte cells (BNL.CL.2) (A) and splenocytes (B) were cultured with various doses of THC in complete medium containing 5% fetal bovine serum for 24 h, and percentage of viable cells was determined by trypan blue exclusion (*, p < 0.05). C, cells were stained by TUNEL for apoptosis and analyzed by flow cytometry. Numbers on histograms indicate percentage of apoptotic cells. D, THC protects hepatocyte cells against apoptosis. BNL.CL.2 cells were preincubated with various doses of THC for 1 h. ConA supernatant [CS; 20% (v/v)] was added, and cells were further cultured for 24 h. Apoptosis was analyzed by TUNEL staining and flow cytometry. Percentage of TUNEL-positive cells is indicated on each histogram.