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. Author manuscript; available in PMC: 2010 Feb 24.
Published in final edited form as: Mol Pharmacol. 2008 Apr 3;74(1):20–33. doi: 10.1124/mol.108.047035

Fig. 5.

Fig. 5

Analysis of regulatory T cells in liver. Hepatitis was induced in mice using ConA as described in Fig. 1. Twelve hours after ConA injection, liver MNCs were analyzed for CD4+CD25+ and CD4+Foxp3+ T cells by double staining. Representative flow profiles with percentage of cell population for each treatment group are shown (A and C) along with absolute cell numbers (B and D). *, p < 0.05 compared with ConA + vehicle. E, 4 h after ConA injection, spleens and livers were collected from each group. cDNA was synthesized from total mRNA, and PCR was carried out using gene-specific primers for Foxp3 and 18S genes.