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. Author manuscript; available in PMC: 2010 Dec 14.
Published in final edited form as: Curr Biol. 2009 Dec 3;19(23):1979–1987. doi: 10.1016/j.cub.2009.10.062

Figure 6. Syp1 directly regulates Las17/WASp.

Figure 6

(A) 4 μM actin (5% pyrene-labeled) was polymerized in the presence of 10 nM yeast Arp2/3 complex and 10 nM yeast Las17/WASp or 20 nM yeast Arp2/3 complex and 200 nM yeast GST-VCA in the presence or absence of 2.5 μM Syp1. (B) Quantification of data from A. Rates of actin assembly for reactions containing Las17/WASp and GST-VCA domain but lacking Syp1 were normalized to 1 for comparative purposes. (C) Control beads or beads coated with Las17/WASp were incubated with soluble Syp1, then pelleted, and the supernatants were analyzed by SDS PAGE and Coomassie staining. (D) Quantification of data in C; levels of Syp1 in the supernatants were measured by densitometry.