Figure 3.

Localization of T-DNAs and Accumulation of CAX1 Transcripts in cax1-3 and cax1-4.

(A) Scheme of the CAX1 gene. Large and small boxes represent exons and introns, respectively. ATG indicates the start codon. T-DNA insertions corresponding to cax1-3 and cax1-4 are shown. The scheme is not drawn to scale.

(B) RNA gel blot hybridization with total RNA (10 μg) obtained from 3-week-old leaves of Col wild-type (WT), cax1-3, and cax1-4 plants grown at 20°C (C) or exposed for 1 day at 4°C (4°C). Equal RNA loading was controlled with a probe for 18S rRNA.