Figure 6.

Freezing Tolerance of cax1-3 and cax1-4.

Three-week-old Col wild-type (WT), cax1-3, and cax1-4 plants were exposed to different freezing temperatures for 1 h. Freezing tolerance was estimated as the percentage of plants surviving each specific temperature after 2 weeks of recovery under unstressed conditions. In (A) and (B), data are expressed as means of three independent experiments with 50 plants each. Bars indicate standard errors.

(A) Freezing tolerance of nonacclimated wild-type and cax1 plants.

(B) Freezing tolerance of cold-acclimated (4°C, 7 days) wild-type and cax1 plants.

(C) Representative cold-acclimated wild-type and cax1 plants after being exposed at −8°C for 1 h and recovering for 2 weeks at 20°C.