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. Author manuscript; available in PMC: 2010 Aug 1.
Published in final edited form as: J Immunol. 2009 Jul 10;183(3):1625–1635. doi: 10.4049/jimmunol.0900339

Figure 1. 7E1-G1 and 7E1-G2b have an equivalent affinity for murine CD40 and effectively inhibit the binding of CD154.

Figure 1

(A) M12 B cells were incubated with the indicated concentrations of 7E1-G1, 7E1-G2b or FGK4.5. Bound antibody was detected with a PE-labeled polyclonal anti-rat IgG secondary antibody by flow cytometry. The values are presented as the percentage of maximum fluorescence observed for each antibody. (B) M12 B cells were incubated with the indicated concentrations of 7E1-G1, 7E1-G2b or FGK4.5 along with 10 μg/mL of Flag-tagged soluble CD154 fusion protein. A FITC-labeled anti-Flag secondary antibody was used to detect bound CD154. The values represent a percentage of the maximum fluorescence observed with sCD154 alone. Data points represent four independent experiments with triplicate samples.