Fig. 1.
Western blot of purified His6-DMGDH. After separation by SDS-PAGE and blotting onto a nitrocellulose membrane, proteins were visualized with anti-DMGDH antibodies. Lanes: (1) purified porcine DMGDH (positive control); (2) purified porcine sarcosine dehydrogenase (negative control); (3) purified His6-DMGDH; and (4) purified flavinated 6His-DMGDH H109R