Skip to main content
. Author manuscript; available in PMC: 2010 Feb 24.
Published in final edited form as: J Inherit Metab Dis. 2008 Oct 21;31(6):761–768. doi: 10.1007/s10545-008-0999-2

Fig. 3.

Fig. 3

Trypsin sensitivity of unflavinated His6-DMGDH H109R. Purified His6-DMGDH H109R (10 μmol/L) with or without bound-FAD was incubated with 0.5 μmol/L trypsin at 37°C for 0 or 240 min. Reactions were stopped by the rapid addition of gel-loading buffer and boiling of the samples. Samples were separated by SDS-PAGE and stained with Coomassie blue dye