Fig. 4.
Plot of the specific activity of DMGDH (0.1 nmol) with dimethylglycine as a substrate, with (squares) or without (diamonds) 4 μmol/L folate. Inset is an Eadie–Hofstee transformation of the data to show the two Km values. DMGDH activity was assayed under standard conditions as described under Materials and Methods. Vertical bars indicate error ranges from triplicate measurements