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. Author manuscript; available in PMC: 2010 Feb 24.
Published in final edited form as: J Inherit Metab Dis. 2008 Oct 21;31(6):761–768. doi: 10.1007/s10545-008-0999-2

Table 1.

Purification of 6His-DMGDH and 6His-DMGDH H109R

Output Protein (mg) Total activity (nmol/min) Specific activity (nmol/min per mg) Yield (%)
His6-DMGDH
 Cells 10 100
 Lysate 814 4 558 5.6 –
 DEAE FF 152 3 025 19.9 66.4
 Ni-NTA 12.7 2 096 165 46.0
His6-DMGDH H109R
 Cells 9 210
 Lysate 854 – –
 DEAE FF 194 – – 60a
 Ni-NTA 4.2 13 3.1 30a
 DEAE FF - 2 0.8 5 6.8 5a
a

Estimated from SDS-gel visualization.