Table 3.
Primers used for amplification and sequencing the ovine BCMO1 and BCO2 genes
| Name | Direction | Position | Sequence5'-3' |
|---|---|---|---|
| BCMO1 - primers | |||
| F0025 | Forward | -109 to -90a | CATCTGAAGGGAGGGAGATG |
| F0066 | Forward | -85 to -66b | AAGACAAGGAGTGGCCAAGA |
| F0157 | Forward | 46-65b | GTGAGGGCCARAGTRACAGG |
| R0673 | Reverse | 520-539b | TTTCCAGCAGCATCGTAGTG |
| F1226 | Forward | 1092-1111b | CGTGGACAAGAATGCAGAAG |
| R1464 | Reverse | 1334-1352b | CTCCTCTCTCCACGTCAAGG |
| R1976 | Reverse | 1826-1845a | GATAGTCCTCACGGCCAAAA |
| BCO2 - primers | |||
| 7541 | Forward | -25 to -6c | CTGCTGCTGCAGAACTCAAC |
| 7542 | Reverse | 648-666d | ATGTGCAGTTGCTCCGTTC |
| 7543 | Forward | 591-611d | GGACATTGAAACCCTGGAAAA |
| 7544 | Reverse | 1392-1404d | CCATTGAATTGGCCATAGTTG |
| 7545 | Forward | 1287-1306d | TTCAGCCAGTGCTGTGAAAC |
| 7546 | Reverse | 1758-1777c | AGCGAGCACATTCATTCAAA |
| 7547 | Forward | -52 to -34c | AGCGTGAGGATTTGGGAAT |
| 7548 | Reverse | 588-607d | CCAGGGTTTCAATGTCCACT |
| 7550 | Forward | 104-126d | TGGAACAGACTCATCAGAAAACA |
| 7551 | Reverse | 257-276d | GAATTTCCCAGGTCCAACAC |
| BCO2F | Forward | 170-189d | TTCTAACCACGGTGGAAGAG |
| BCO2R | Reverse | 230-249d | ATAGCCATTGAGCCACTCAG |
| BCO2E | Forward | 177-195d | CACGGTGGAAGAGACTCTG |
a. Positions are numbered according to first base of the translation start codon of the bovine BCMO1 gene (NM_001024559.1).
b. Positions are numbered according to first base of the translation start codon of the ovine BCMO1 gene (FN543098)
c. Positions are numbered according to first base of the translation start codon of the bovine BCO2 gene (NM_001101987).
d. Positions are numbered according to first base of the translation start codon of the ovine BCO2 gene (FN257486).