Figure 3.

phyA Controls PKS1 and PKS2 Expression.

(A) RNA gel blot analysis of 5-day-old dark-grown wild-type seedlings transferred for 1, 2, 4, or 18 h to continuous R or FR and probed with PKS1, PKS2, and ACT probes. D, darkness.

(B) RNA gel blot analysis of 5-day-old dark-grown phyA-211 seedlings treated and probed as in (A).

(C) RNA gel blot analysis of 3-day-old dark-grown wild-type seedlings treated with one, two, or four 3-min FR pulses at 1-h intervals and a pks1, pks2 mutant treated with two FR pulses as a control. The membrane was probed with PKS1 and ACT as a loading control. Col, Columbia wild type.