Table 2.
Oligonucleotide primers used for cDNA cloning or RT--PCR analysis
| Gene (accession number) | Primer | Sequence | Position* |
|---|---|---|---|
| ovSOLD1 and caSOLD1 | Forward | 5'--TCCAGAGATGGCTAAGTGCCTT--3' | 50--71 |
| (NM_001105478) | Reverse | 5'--GAGTTGGACATGACTGAGCCAC--3' | 453--432 |
| ovGAPDH | Forward | 5'--AAGGCCATCACCATCTTCCA--3' | 78--97 |
| (AF030943) | Reverse | 5'--AGGTCAGATCCACAACGGACA--3' | 603--583 |
| caGAPDH | Forward | 5'--GACCCCTTCATTGACCTTCAC--3' | 1--21 |
| (AJ431207) | Reverse | 5'--TCATAAGTCCCTCCACGATGC--3' | 424--404 |
* The nucleotide position in each accession number