Figure 2. Modulation of cytokine production by HDL in CEsHUT-activated monocytes.
Monocytes (5×104 cells/200 µl/well; 96-well plates) were activated by CEsHUT for 24 h in the presence (empty columns) or absence (grey columns) of HDL. Cell culture supernatants were tested for the presence of the indicated cytokines by BioPlex using a 27-Plex kit. Results were obtained from monocytes isolated from 3 individual donors and are expressed as mean ± SD (n = 3). (A) Cytokines previously shown to be modulated by CEsHUT; (B) CEsHUT-induced cytokines whose expression was not modulated by HDL; and (C) CEsHUT-induced cytokines whose expression was inhibited in the presence of HDL.
