Figure 3. Inhibition by HDL of CEsHUT-triggered IL-1β mRNA.
Monocytes (7×106 cells/P-100mm/6 ml) isolated from 3 different donors (a, b and c) were activated by CEsHUT (6 µg/ml) in the presence (empty columns) or absence (grey columns) of HDL (0.2 mg/ml) for 3 h. (A) Isolated RNA was subjected to qPCR to assess IL-1β transcript levels. Results are presented as mean ± SD of triplicates. (B) Isolated RNA was subjected to microarray analysis. Results are expressed as mean ± SD, n = 3.
