Figure 6. Holographic photostimulation of NG2 cells.

A. Red fluorescence image of a target DsRed⁺ NG2 cell (arrowhead) in a hippocampal slice of NG2-DsRed transgenic mice. B. Photolysis-evoked currents elicited in the same cell held at −70 mV upon illumination with a 5 µm spot (a) and shaped pattern (b), using the same power density (120 nJ/µm²). Individual sweeps (gray) and averaged currents (red and black) are shown. C. Normalized photolysis-evoked currents shown in (B). Note the similarity of the kinetics. D. Fluorescence images of a target DsRed⁺ NG2 cell in a OGB-loaded hippocampal slice of NG2-DsRed transgenic mice (arrowheads). E. Variation of intracellular Ca²⁺ concentration during photostimulation with a 5 µm spot (a) and shaped pattern (b), using the same power density (100 nJ/µm²). Scale bars for insets: 10 µm.