The effect of diabetes, insulin, or its combination with IGF on the relative levels of the ubiquitous proteins actin, α-tubulins, and β-tubulins per brain. SDS/urea 10,000×g supernatant brain extracts were subjected to SDS-PAGE and Western blots. The upper panels shows representative bands on Western blots, and the lower panels show group means ± s.e.m. Lane 1, Non-D; Lane 2, D+aCSF; Lane 3, D+Ins; and Lane 4, D+Ins+IGF. A. Relative pan-actin levels per brain. B. Pan α-tubulin levels per brain. C. Pan β-tubulin levels per brain.