Figure 1. 1,25D inhibits cell proliferation in Multipotent mesenchymal C3H 10T1/2 in a dose dependent manner.
(A) Cells treated with or without 1,25D (100 nM) for 4 days were fixed with 2% p-formaldehyde and counterstained with hematoxyline (200×). (B) C3H 10T1/2 cells primed with 5′-azacytidine (20 μM) at an initial number of 4,000 cells/well were incubated for 4 days, as follows: control: no addition of 1,25D and 1,25D: increasing concentrations of 1,25D from 10 – 500 nM. Results are expressed as Mean±SEM. *P<0.05; ***P<0.001