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. Author manuscript; available in PMC: 2011 Mar 1.
Published in final edited form as: J Steroid Biochem Mol Biol. 2010 Jan 11;119(1-2):73–83. doi: 10.1016/j.jsbmb.2010.01.001

Figure 3. 1,25D decreases cell number and increases cell size of multipotent mesenchymal cells.

Figure 3

Cells treated as in Figure 1 were preincubated with Green Fluorescent Cell Linker (PKH2) and counterstaing with DAPI. Next day, cells were incubating with or without 1,25D for 4 and 7 days respectively. Representative pictures are presented for control (No 1,25D addition) and 1,25D (100nM) at 4 days (A upper panel) and at 7 days (B, upper panel) with the corresponding histograms showing the cell size distribution and expressing the number of cells per area (μm2). (A and B, lower panels)