Table 3. Confirmation of the effects of 1,25D on the transcriptional expression of cell cycle related genes in C3H 10T1/2 multipotent cells by real-time PCR arrays.
Total RNA from cells treated as in Table 2 was subjected to RT-real time PCR by the RT2 profiler PCR mouse Cell cycle array and the ratios between the 1,25D treated and 1,25D-untreated cells corrected by GAPDH were calculated for the assays performed in triplicates.
| Symbol | Description | Real time PCR Array Ratios (4 days) |
|---|---|---|
| Ccna2 | Cyclin A2 | -4.39 |
| Ccnb1 | Cyclin B1 | -4.87 |
| Ccnb2 | Cyclin B2 | -3.44 |
| Ccnd1 | Cyclin D1 | -2.85 |
| Ccne1 | Cyclin E1 | -3.21 |
| Ccnf | Cyclin F | -4.40 |
| Cdk2 | Cyclin-dependent kinase 2 | -2.05 |
| Cdk4 | Cyclin-dependent kinase 4 | -2.25 |
| Chek1 | Checkpoint kinase 1 homolog (S. pombe) | -3.0 |
| Skp2 | S-phase kinase-associated protein 2 (p45) | -2.60 |