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. Author manuscript; available in PMC: 2011 Mar 1.
Published in final edited form as: Cornea. 2010 Mar;29(3):321–330. doi: 10.1097/ICO.0b013e3181b1165d

Figure 3.

Figure 3

A-F: Corneal epithelial knockin (KI) cell lines. Recombinant viruses were generated by cotransfection of 293T cells with EGFP-expressing recombinant pFB vectors coding either MT1-MMP or MT1-MMP-E240A. High-titer recombinant viruses containing medium amplified in 293T cells were used to infect MT1-MMP KO epithelial cell lines with MT1-MMP or MT1-MMP-E240A. Each transfected cell line (KO Epi/MT1-MMP (KI) or KO Epi/MT1-MMP-E240A (KI)) was sorted based on EGFP expression using flow cytometry. Stably-transfected epithelial cell lines were generated and confirmed by fluorescence microscopy: KO Epi/MT1-MMP (KI) (Fig. 3A, B) and KO Epi/MT1-MMP-E240A (KI) (Fig. 3C, D). E: Expression of MT1-MMP (E, lane 1) and MT1-MMP-E240A (E, lane 2) in MT1-MMP KO epithelial cell lysates was determined by western blot. F: Expression of MT1-MMP (F, lane 1) and MT1-MMP-E240A (F, lane 2) was also assayed by western blotting of conditioned media.