Figure 1.

Conditional Deletion of Cited2 Results in Multilineage Bone Marrow Failure

(A) Relative expression of Cited2 mRNA in LT-HSC, ST-HSC, and LMPP populations sorted from WT C57BL/6J mice. Data are mean ± SEM (n = 3).

(B) Cited2^fl/flMx1-Cre and Cited2^fl/fl mice received six injections of pIpC on alternate days and analyzed 5 days after the last injection.

(C) Relative expression of Cited2 mRNA in total BM cells from Cited2^Δ/Δ and control mice (mean ± SEM; n = 3).

(D) Total number of BM nucleated cells obtained from two tibias and two femurs of Cited2^Δ/Δ and control mice. The results are presented as mean number of cells ± SD (n = 5). ^∗p < 0.0001.

(E) Top and middle: Frequencies of B-lymphoid and myeloid cells, respectively, in BM from Cited2^Δ/Δ and control mice. Bottom: FACS plot showing CD4 and CD8 staining in thymi from Cited2^Δ/Δ and control mice. Data are shown as mean frequency ± SD (n = 3).