Figure 4.

Pti4 Interacts in Vivo with Both GCC Box– and Non-GCC Box–Containing Promoter Sequences.

Gels show results from the 18 promoters that were analyzed in the ChIP assay (see Table 4 for summary). The names of the promoters analyzed are given above the gels; MYB73 was used as a negative control. Lanes contain PCR products from promoter-specific primers obtained from genomic DNA or chromatin extract. Inputs for PCR amplification were as follows: 1, genomic DNA from wild-type Arabidopsis; 2, genomic DNA from Pti4-expressing Arabidopsis; 3, chromatin immunoprecipitated with preimmune serum from Pti4-expressing Arabidopsis; 4, chromatin immunoprecipitated with Pti4 antibody without cross-linking from Pti4-expressing Arabidopsis; 5, chromatin immunoprecipitated with Pti4 antibody from wild-type Arabidopsis; and 6, chromatin immunoprecipitated with Pti4 antibody after cross-linking from Pti4-expressing Arabidopsis. The presence of a PCR product in lane 6 (asterisks) indicates binding by Pti4. A 1-kb DNA size ladder (M) from New England Biolabs (Beverly, MA; top gels) and a 100-bp ladder from Amersham (middle and bottom gels) are labeled.