Table 1.
Common methods to cleave peptides at specific amino acid sequences
| Cleavage agent | Cleavage specificity |
|---|---|
| Chemical | |
| Hydroxylamine | -N ↓ G- |
| Cyanogen bromide | -M ↓ X- |
| Formic acid | -D ↓ P- |
| 2-iodosobenzoic acid | -W ↓ X- |
| 3-bromo-3-methyl-2-(2-nitrophenylthio)-3H-indole | -W ↓ X- |
| 2-nitro-5-thiocyanatobenzoic acid | -C ↓ X- |
| 1-cyano-4-dimethylaminopyridiumtetrafluoroborate | -C ↓ X- |
| Enzymatic | |
| Enterokinase | -DDDDK ↓ X- |
| SUMO protease | SUMO-GG ↓ XXX- |
| TEV protease | -ENLYFQ ↓ (S,G)- |
| Factor Xa | -IDGR ↓ X- |
| Thrombin | -LVPR ↓ GS- |
| HRV 3C protease | -LEVLFQ ↓ GP- |
| IGase | -PP ↓ YP- |
| Furin | -RX(R/K)R ↓ X- |
| Endoproteinase Lys-C | -K ↓ X- |
| Endoproteinase Glu-C | -E ↓ X- |
| Endoproteinase Arg-C | -R ↓ X- |
| Endoproteinase Asp-C | -D ↓ X- |