Figure 7.

Summary of How Microtubules Are Generated at Kinetochores, How They Facilitate Kinetochore Interaction with Spindle Pole Microtubules, and How They Disappear Afterwards, in Budding Yeast

(A) MTs are generated at KTs with distal plus ends. Stu2 has a central role in promoting this process at KTs. This MT generation is more frequent when the KT interaction with spindle pole MTs is delayed.

(B) KT-derived MTs interact with spindle pole-derived MTs. This interaction takes place either in an (i) antiparallel or (ii) parallel manner, and facilitates KT loading onto the lateral side of spindle pole MTs.

(C and D) After KTs are loaded onto the lateral side of spindle pole MTs, the amount of Stu2 at KTs is reduced and new MT generation at KTs is suppressed. Existing KT-derived MTs shrink and quickly disappear.

(E) KTs subsequently attach to the distal plus ends of spindle pole MTs (Tanaka et al., 2007).