Table 1.
Transduction and Engraftment of Canine CD34+ Cells
| Dog | Vector | No. of CD34-enriched cells (× 106) before culture | Purity of CD34-enriched cells | MOI | In vitro markinga | No. of infused cells (× 106) | Days to ANC >500/μl | Days to platelets >20,000/μl |
|---|---|---|---|---|---|---|---|---|
| G380 | Foamy virus | 20 | 86.5% | 5 | 11.6% | 9.0 | 10 | 23 |
| Lentivirus | 20 | 86.5% | 5 | 4.4% | 20.0 | 10 | 23 | |
| G480 | Foamy virus | 18 | 86% | 5 | 14% | 9.6 | 14 | 11 |
| Lentivirus | 18 | 86% | 5 | 9.5% | 15.6 | 14 | 11 |
Abbreviations: ANC, absolute neutrophil count; MOI, multiplicity of infection.
a
Percentage of EGFP (foamy)- or EYFP (lenti)-positive cells in transduced CD34-enriched cultures 10 days after vector exposure.