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. 2009 Dec 10;59(3):564–571. doi: 10.2337/db09-0997

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© 2010 by the American Diabetes Association.

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FIG. 3. — Expression and release of IL-6, MCP-1, and adiponectin into conditioned media after siRNA treatment of cultured adipocytes. Human in vitro–differentiated adipocytes were treated with 50 nmol/l control siRNA or siRNA directed against twist1. Expression of the inflammatory genes IL-6, TNF-α, and MCP-1 as well as adiponectin was measured after siRNA treatment (A). mRNA levels were normalized to the reference gene GAPDH. Release of IL-6 (B), MCP-1 (C), and adiponectin (D) was measured in conditioned media; n = 4 (IL-6) and n = 3 (MCP-1/adiponectin). Results shown are mean ± SEM and mRNA levels are shown as relative expression (fold change to control siRNA). **P < 0.01, *P < 0.05.