FIG. 2.
Glucose tolerance test and lipid levels in RLIP76−/− mice. For the glucose tolerance test, C57B mice (wild-type and RLIP76−/−) were fasted for 6 h followed by oral administration of 2 g/kg body wt glucose using a gavage needle. Each of five groups consisted of five animals each. One group was killed at each time point (0, 5, 30, 60, and 120 min) and ∼200–300 μl of blood was sampled for glucose analyses. Blood was kept refrigerated for ∼4 h and centrifuged at 3,000g for 10 min, and the serum was separated and stored at −20°C until assay. Glucose, cholesterol, and triglycerides measurement was performed in the laboratory of Dr. Kent R. Refsal, Michigan State University. *Statistical analyses by ANOVA were significant at P < 0.001 for RLIP76−/− vs. RLIP76+/+ animals; n = 5.