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. 2009 Dec 10;59(3):714–725. doi: 10.2337/db09-0911

FIG. 3.

FIG. 3.

Insulin-to-glucose ratio in RLIP76-deficient and -supplemented mice. Twelve-week-old C57BL/6 mice born of heterozygous × heterozygous mating were genotyped by PCR. Insulin and glucose measurement in blood serum was performed on wild-type (RLIP76+/+) animals killed 24 h after a single intraperitoneal injection of control or RLIP76 liposomes equivalent to 200 and 500 μg RLIP76 protein. Insulin and glucose measurement in blood serum was also performed on heterozygous (RLIP76+/−) and homozygous (RLIP76−/−) animals. The values are presented as means ± SD from three separate determinations with three replicates (n = 9) (A and B). Western blot analyses for RLIP76 were performed on liver and heart tissues of RLIP76+/+ male and female mice killed 24 h after administration of 200 or 500 μg RLIP76 protein in the form of liposomes, intraperitoneally. Aliquots of 100 μg detergent-solubilized crude membrane fraction from the liver and heart of animals treated with 200 μg (R2) or 500 μg (R5) were loaded per lane in SDS-PAGE, transblotted, and probed using anti-RLIP76 IgG primary and peroxidase-conjugated goat anti-rabbit IgG secondary antibody. The blots were developed with 4-chloro-1-napthol as chromogenic substrate (A and B insets). β-actin expression was used as loading control. Statistical analyses by ANOVA were significant at P < 0.01 for RLIP76−/− vs. RLIP76+/−, RLIP76−/− vs. RLIP76+/+, RLIP76+/− vs. RLIP76+/+, and RLIP76+/+ vs. RLIP76 proteoliposome–treated RLIP76+/+.

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