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. 2009 Dec 3;59(3):600–610. doi: 10.2337/db09-0897

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© 2010 by the American Diabetes Association.

Readers may use this article as long as the work is properly cited, the use is educational and not for profit, and the work is not altered. See http://creativecommons.org/licenses/by-nc-nd/3.0/ for details.

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FIG. 5. — Effect of OKA on PKB/Akt phosphorylation in non-CEMs. A: 3T3-L1 adipocytes were treated with 100 μmol/l ceramide for 2 h and/or with 500 nmol/l OKA for the last 30 min. Equal amounts of protein (1 μg) of the non–DRM-containing fractions (10–12) were then immunoblotted with antibodies against native PKB/Akt and Ser473 PKB/Akt. B: 3T3-L1 adipocytes were treated with 100 μmol/l ceramide for 2 h and with 500 nmol/l OKA for the last 30 min, followed by insulin for the last 10 min. Equal amounts of protein (1 μg) of the non–DRM-containing fractions (10–12) were then immunoblotted with antibodies against native PKB/Akt and Ser473 PKB/Akt. These are representative of at least three independent experiments.