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. Author manuscript; available in PMC: 2011 Feb 19.
Published in final edited form as: Biochem Biophys Res Commun. 2010 Jan 25;392(4):593–598. doi: 10.1016/j.bbrc.2010.01.084

Fig. 3.

Fig. 3

Reduced Notch activity in 14-3-3σ mutant-expressing corneal epithelial cells. (A) Western blots showing the level of Notch1 NICD in corneal epithelial cells transduced with the control GFP-lentivirus (WT, 1–4) or with lentivirus expressing the 14-3-3σ mutant cDNA (Mut, 5–8) in low calcium (lanes 1, 2, 5, and 6) or high calcium (lanes 3, 4, 7, and 8). (B) Q-PCR analysis of Hes1, RBP1, Notch1, Notch2, Jagged1, and Jagged2 mRNAs in corneal epithelial cells transduced with lentivirus expressing either GFP or mutant 14-3-3σ.