Reduced Notch activity in 14-3-3σ mutant-expressing corneal epithelial cells. (A) Western blots showing the level of Notch1 NICD in corneal epithelial cells transduced with the control GFP-lentivirus (WT, 1–4) or with lentivirus expressing the 14-3-3σ mutant cDNA (Mut, 5–8) in low calcium (lanes 1, 2, 5, and 6) or high calcium (lanes 3, 4, 7, and 8). (B) Q-PCR analysis of Hes1, RBP1, Notch1, Notch2, Jagged1, and Jagged2 mRNAs in corneal epithelial cells transduced with lentivirus expressing either GFP or mutant 14-3-3σ.