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. Author manuscript; available in PMC: 2011 Feb 19.
Published in final edited form as: Biochem Biophys Res Commun. 2010 Jan 25;392(4):593–598. doi: 10.1016/j.bbrc.2010.01.084

Fig. 4.

Fig. 4

Defective mutant 14-3-3σ-expressing corneal epithelial cell differentiation can be rescued by expression of Notch1 NICD. (A). Wild-type (columns 1 and 2) or mutant 14-3-3σ-expressing (columns 3 and 4) corneal epithelial cells were transduced with NICD-expressing (columns 2 and 4) or GFP--expressing (columns 1 and 3) lentivirus (One day after transduction, the cells were switched to high calcium medium for 48 h, then Hes1 mRNA levels were assessed by Q-PCR. (B) 14-3-3σ mutant-expressing corneal epithelial cells were transduced by lentiviral GFP-expressing (top) or NICD/GFP expressing (bottom) vectors in high calcium medium for 48 h, then tight junction (TJ) formation was visualized by staining with anti-ZO-1 antibody. GFP or NICD-GFP lentiviral expression were showed in green (left). Bar size=100μm. (C) TJ-positive cells were quantified and the fold increase in TJ-positive cells in the NICD-expressing cells was calculated by comparison with the GFP-expressing controls.